Electroimmunoassays consists of a circuit with a capture antibody which is attached to the solid surface of the electrode gap area. This technology to isolate and identify foodborne pathogens combines specific antibody-antigen binding to the production of an electrical signal. The target antigen binds to the capture antibody when the sample is added. Following that, a colloidal gold–labeled detection antibody is bound to create a capture-target-detector sandwich. Finally, the silver ions are deposited onto the colloidal gold, which produces a conductive silver bridge hence closing the circuit and resulting in a measured drop in resistance.
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